Nematic orders are highest in the lumen of the NPC. (A) Nematic order parameters calculated inside and outside of the NPC lumen, averaged over four datasets of 100 ms. With decreasing FG-Nup–binding strength, increased nematic order within the NPC is observed. (B) Nematic order parameters were calculated across the nuclear–cytoplasmic axis (x axis is in nanometers) and averaged over four datasets of 100 ms. All plots are mean ± SD.