The orientational rigidity of FG-Nups exhibit differential dependence on FG–FG bond strength. Autocorrelation plots of filaments, taken at three locations along filament length, base, middle, and tip. (A–D) FG-Nup116, (E–H) FG-Nup57, and (I–L) FG-Nup159, each at four different FG–FG off rates (FG4, FG5, FG6, and FG_off). Plotted lines represent mean of autocorrelation functions ± standard error. Blue, tip segment; green, middle segment; red, base segment.