Figure 9.
Figure 9. A kinase-dead mutant of tPA still slows PAI-pHl secretion. Bovine chromaffin cells were cotransfected to express PAI-pHl in tandem with tPA, S513A tPA, or empty vector control (pcDNA3). Secretion was stimulated with 56 mM potassium buffer and observed by TIRF microscopy at a rate of 36 Hz. pHl fluorescence intensity was analyzed with a custom program as described in Fig. 5. (A) Each dot represents one fusion event. Medians are indicated by lines. One-way ANOVA was performed with a post hoc Kruskal–Wallis test. (B) Cumulative histogram plots data from A.

A kinase-dead mutant of tPA still slows PAI-pHl secretion. Bovine chromaffin cells were cotransfected to express PAI-pHl in tandem with tPA, S513A tPA, or empty vector control (pcDNA3). Secretion was stimulated with 56 mM potassium buffer and observed by TIRF microscopy at a rate of 36 Hz. pHl fluorescence intensity was analyzed with a custom program as described in Fig. 5. (A) Each dot represents one fusion event. Medians are indicated by lines. One-way ANOVA was performed with a post hoc Kruskal–Wallis test. (B) Cumulative histogram plots data from A.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal