Figure 3. Effect of D933N on the mutants’ leak currents. (A) Continuous TEVC recording at −50 mV from an oocyte expressing V332G/D933N. (B) Mean Iouab in NMG+o (filled symbols) and Na+o (open symbols) for double mutants L104R/D933N (down triangles), V332G/D933N (circles), delF100-L104/D933N (diamonds), and EETA963S/D933N (up triangles), recorded 3–4 d after injection. Note axis break at negative currents caused by larger currents in oocytes expressing delF100-L104/D933N. The number of experiments is indicated in parentheses. Error bars represent SEM. (C) Western blot of protein recognized by the Anti-KETYY antibody targeting the C-terminal end of the Na/K-ATPase. Left lane shows purified sheep kidney enzyme (0.5 µg total protein) and a membrane preparation from 25 oocytes injected with Xenopus RD-α1-EETA963S/D933N cRNA (20 µg total protein). Bands at ∼110 kD (the approximate mass of the Na/K-ATPase α-subunit) are visible for both samples.
Figure 3.

Effect of D933N on the mutants’ leak currents. (A) Continuous TEVC recording at −50 mV from an oocyte expressing V332G/D933N. (B) Mean Iouab in NMG+o (filled symbols) and Na+o (open symbols) for double mutants L104R/D933N (down triangles), V332G/D933N (circles), delF100-L104/D933N (diamonds), and EETA963S/D933N (up triangles), recorded 3–4 d after injection. Note axis break at negative currents caused by larger currents in oocytes expressing delF100-L104/D933N. The number of experiments is indicated in parentheses. Error bars represent SEM. (C) Western blot of protein recognized by the Anti-KETYY antibody targeting the C-terminal end of the Na/K-ATPase. Left lane shows purified sheep kidney enzyme (0.5 µg total protein) and a membrane preparation from 25 oocytes injected with Xenopus RD-α1-EETA963S/D933N cRNA (20 µg total protein). Bands at ∼110 kD (the approximate mass of the Na/K-ATPase α-subunit) are visible for both samples.

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