Location of hyperaldosteronism mutations. Zoomed-in view of the ion-binding sites in the E1(3Na) pig Na/K pump structure (Protein Data Bank accession no. 2ZXE) indicating several ion-coordinating residues. The three Na⁺ ions bound are shown in purple, and the carbon backbone of residues altered by hyperaldosteronism-associated Na/K pump mutations studied here is the same color scheme used for each mutant throughout the article.