Co–first authors Akira Uehara (left) and Takashi Murayama (middle) and senior author Nagomi Kurebayashi (right), together with co–first author Midori Yasukochi and others (not shown), demonstrate how a disease-causing mutation at lysine 4750 affects gating of human ryanodine receptor 2. Residue 4750 is located on the channel’s cytoplasmic face, near its transmembrane pore (highlighted in pink in this top-down view ribbon diagram). Photos courtesy of the authors.