Figure 5.
Figure 5. Buffer effects on Na+ binding enthalpy (ΔHITC(Na+)). ITC was used to determine Na+ binding ΔHITC(Na+) to MelBSt in a total of five buffer systems in the absence of melibiose at pH 6.25, 7.45, and 8.2 at 25°C. 5–15 mM NaCl was placed in the syringe. (a) ΔG versus standard buffer −ΔH(H+) plot. (b) ΔHITC(Na+) determined by ITC versus buffer standard −ΔH(H+) plot, with linear fits to the data.

Buffer effects on Na+ binding enthalpy (ΔHITC(Na+)). ITC was used to determine Na+ binding ΔHITC(Na+) to MelBSt in a total of five buffer systems in the absence of melibiose at pH 6.25, 7.45, and 8.2 at 25°C. 5–15 mM NaCl was placed in the syringe. (a) ΔG versus standard buffer −ΔH(H+) plot. (b) ΔHITC(Na+) determined by ITC versus buffer standard −ΔH(H+) plot, with linear fits to the data.

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