Figure 4.
![Figure 4. Changes in SL and local [Ca2+] during spontaneous beating at different temperatures. (A) Time course of changes in SL and local Fyellow/Fcyan measured with α-actinin–YC-Nano140 during spontaneous beating at 37°C. Data were averaged from 10 sarcomeres. (B) Same as in A at 22°C. Data were averaged from 13 sarcomeres. (C) Graph summarizing time to peak values of changes in SL and Fyellow/Fcyan at 22°C and 37°C. *, P < 0.05; and ***, P < 0.001 (Tukey-Kramer test). (D) Graph comparing delay time (difference in time between SL and Fyellow/Fcyan) at 22°C and 37°C. ***, P < 0.001 (Mann-Whitney U test). Bars in each graph indicate mean values.](https://cdn.rupress.org/rup/content_public/journal/jgp/148/4/10.1085_jgp.201611604/5/jgp_201611604_fig4.jpeg?Expires=1767805654&Signature=SCSMev19mhszY5SvOZ5LEakxzuhoN3UweDDdr~gqyzg2DozBf~heT4mO5-KdzJLcdUVXj8tZAzVuerDI3yyz9VahFR2MPf4hp8OXv28od8ZDIGjpbOhmCCwd4NvyoABrHyxObp1O9x9X8NSNHZVneCmOSfi5u70l5BB9F~54iXyRF48VbG9pstiD5VSnKOBXezPoPVP5okPiJJpN7GoTJQYpdCzx7uyTRZptSk~tlpAJDwPb6l8BOgJgbsmhGXXwzc6Ww5S6CS1vzKymmXW5yg7etZaKi0~1N2L2uGIitiTwxQOJL6XuIy0Vf97N5pQ-s~RTCH-ydf8QnXPaV1UCAw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Changes in SL and local [Ca2+] during spontaneous beating at different temperatures. (A) Time course of changes in SL and local Fyellow/Fcyan measured with α-actinin–YC-Nano140 during spontaneous beating at 37°C. Data were averaged from 10 sarcomeres. (B) Same as in A at 22°C. Data were averaged from 13 sarcomeres. (C) Graph summarizing time to peak values of changes in SL and Fyellow/Fcyan at 22°C and 37°C. *, P < 0.05; and ***, P < 0.001 (Tukey-Kramer test). (D) Graph comparing delay time (difference in time between SL and Fyellow/Fcyan) at 22°C and 37°C. ***, P < 0.001 (Mann-Whitney U test). Bars in each graph indicate mean values.
