Ethanol modulates parameters associated with Ca²⁺_i binding affinity of cbv1+β1 channels. (A and B) The averaged (log [R₀])-[Ca²⁺_i] plots (voltage: −120 mV) in the absence (A) and presence (B) of 50 mM ethanol. (log [R₀])-[Ca²⁺_i] plots were fitted with the Eq. 5. Best-fit parameters (±95% confidence interval) are shown to the left of the corresponding plots. Data demonstrate that ethanol significantly decreases the values of K_d and C. n = 4–5; each patch was excised from a different cell. Data are expressed as mean ± SEM.