Ethanol does not alter the voltage-dependent parameters of cbv1+β1 channels. (A–C) Representaive current traces (A) and mean log Po-V relations in the absence (B) and presence (C) of 50 mM ethanol. At voltages less positive than 80 mV, data points were obtained from unitary current recordings; at voltages more positive than 80 mV, data points were obtained from macroscopic current recordings obtained at nominal zero Ca²⁺_i. Po-V data obtained over a wide range of voltages were fitted with Eq. 2 to determine the values of V_h(J), L₀, z_L, D, and z_J. For control data, V_h(J) was constrained to 80 mV (according to Bao and Cox [2005]), whereas for ethanol data V_h(J) was allowed to vary. Best-fit parameters (±95% confidence interval) are listed in the corresponding graphs. n = 12; each patch was excised from a different cell. Data are expressed as mean ± SEM.