Ethanol does not alter the allosteric interaction between Ca²⁺ binding and voltage sensor activation of cbv1 channels. (A and B) Averaged G/G_max-V curves obtained over a wide range of Ca²⁺_i in the absence (A) and presence (B) of 50 mM ethanol. G/G_max-V plots were fitted with Eq. 1. For curves in panel A, L₀, z_L, V_h(J), z_J, D, K_d, and C were constrained to 2.3 × 10⁻⁶, 0.34, 155, 0.6, 19.8, 9.02, and 6.07, respectively (Figs. 7, 8, and 9), whereas E was allowed to vary. For G-V curves in panel B, L₀, z_L, V_h(J), z_J, D, K_d, and C were fixed to 2.1 × 10⁻⁶, 0.35, 155.1, 0.6, 19.6, 1, and 4.7, respectively, whereas E was allowed to vary. Best-fit parameters (±95% confidence interval) are shown to the left of the corresponding plots. n = 4–8; each patch was excised from a different cell. Data are expressed as mean ± SEM.