Functional properties of the WT_WT concatemer. (A) Representative currents of the WT_WT concatemer at 80 and −80 mV. Blue and red dots indicate the reference pulses used for rundown correction at 80 and −80 mV, respectively (see Materials and methods). (B) Rundown-corrected concentration–response relations of the WT_WT concatemer at 80 and −80 mV (n = 8–11 for each data point). Lines are the best fit to a biphasic Hill equation. Dashed lines indicate the relations of WT mTMEM16A at 80 and −80 mV. (C) Na⁺ versus Cl⁻ selectivity of the WT_WT concatemer. (left) I-V plots recorded at 150 mM extracellular and the indicated intracellular NaCl concentrations at 2 µM (n = 6) [Ca²⁺]_free. (right) Relation between intracellular NaCl concentration and E_rev at 2 µM (n = 6) [Ca²⁺]_free. The line indicates the Nernst potential of Cl⁻. (D, left) Merged and averaged variance-<I> plots of the WT_WT concatemer obtained at 2 µM (n = 5) [Ca²⁺]_free at 80 mV. The dotted line indicates the mean P_{O max} across patches. (right) Pooled data of individual i and P_{O max} values obtained from individual patches expressing the WT_WT concatemer at 2 µM (n = 5) [Ca²⁺]_free. Dotted lines indicate the corresponding value of WT mTMEM16A. In B–D, data are presented as mean and errors as SEM.