Figure 2. Purification and biochemical... | Rockefeller University Press

Figure 2.

$Figure 2. Purification and biochemical characterization of mTMEM16 constructs. (A) Gel filtration profiles of WT and the WT_E702Q concatemer. WT (top) and WT_E702Q (center) loaded after purification in digitonin. The protein was detected by the fluorescence of the YFP tag. The bottom graph shows a reinjection of the main peak fraction of WT_E702Q. The elution volume is indicated. LU, luminescence units. (B) SDS-PAGE of the purified proteins. Both proteins (WT and WT_E702Q) run at the expected molecular weight. Molecular weights of the markers (kilodaltons) are indicated.$

Purification and biochemical characterization of mTMEM16 constructs. (A) Gel filtration profiles of WT and the WT_E702Q concatemer. WT (top) and WT_E702Q (center) loaded after purification in digitonin. The protein was detected by the fluorescence of the YFP tag. The bottom graph shows a reinjection of the main peak fraction of WT_E702Q. The elution volume is indicated. LU, luminescence units. (B) SDS-PAGE of the purified proteins. Both proteins (WT and WT_E702Q) run at the expected molecular weight. Molecular weights of the markers (kilodaltons) are indicated.