Spontaneous activity in OSNs from I7-GFP WT and KO mice for TMEM16B. (A and B) Loose-patch recordings (A; 60-s recordings) and raster plots (B) of the spontaneous firing activity from three WT OSNs (top) and three KO mice (bottom). (C and E) Cumulative plot of spontaneous mean (C) and instantaneous (E) firing frequency, bin of 0.1 Hz, in control (black lines) and TMEM16B KO mice (blue lines). Mann-Whitney U test, one-tail: **, P < 0.01 in C; and ***, P < 0.001 in E. (D and F) Box and scatter plots of spontaneous mean (D) and instantaneous (F) firing activity in OSNs in WT and KO mice. In the box plots, the inner squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. (G) ISI distribution histogram (bin = 5 ms) normalized to the number of recorded neurons in control (black line, n = 34 from 19 mice) and KO mice (blue line, n = 22 from 9 mice).