Spontaneous activity in OSNs in WT and KO mice. (A and B) Loose-patch recordings (A; 60-s recordings) and raster plots (B) of the spontaneous activity in three WT OSNs (top) and three KO OSNs (bottom). (C and E) Cumulative plot of spontaneous mean (C) and instantaneous (E) firing frequency, bin of 0.1 Hz, in control (black lines) and TMEM16B KO mice (blue lines). Mann-Whitney U test, one-tail: P > 0.05 for C; *, 0.01 < P < 0.05 for E. (D and F) Box and scatter plots of spontaneous mean (D) and instantaneous (F) firing activity in OSNs in WT and KO mice. In the box plots, the inner squares represents the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. (G) ISI distribution histogram (bin = 5 ms) normalized to the number of recorded neurons in WT (black line, n = 106 from 47 mice) and KO mice (blue line, n = 52 from 22 mice).