Evoked activity in OSNs in I7-GFP WT and KO mice for TMEM16B. (A and B) Representative recordings of responses to a puff of 50 ms of 500 µM heptanal for two different OSNs from WT (A) and KO mice (B). The bottom panels show the regions enclosed by the dashed boxes on an expanded time scale. Broken red lines represent the onset of heptanal stimulation, and the red bars in the bottom panels its duration. (C and D) Raster plots on an enlarged time scale for the same cells shown in A and B. (E) Averaged responses to the same odorant puff as A and B, normalized to the number of neurons (n = 13 from 7 WT mice, n = 9 from 5 KO mice; bin 300 ms). The broken red line represents the onset of stimulation, and data belong to WT and KO PSTH distributions. *, 0.01 < P < 0.05; and **, P < 0.01. (F–H) Scatter plot of ISI of the first four spikes (F; 23 ± 13 ms in WT, 20 ± 11 ms in KO; mean ± SD; Mann-Whitney U test, one tail: P > 0.05), of the total number of spikes (G; 12 ± 10 spike/response in WT, 70 ± 57 spike/response in KO; Mann-Whitney U test, one tail: **, P < 0.01), and of the response duration (H; 2.3 ± 1.3 s in WT, 8.1 ± 7.0 s in KO; Mann-Whitney U test, one tail: **, P < 0.01). In the box plots the squares represent the mean, lines represent the median, upper and lower box boundaries represent the 25th and 75th percentile, and upper and lower whiskers represent the 5th and 95th percentiles. The number of experiments is indicated in parentheses.