Transport characteristics and membrane expression of AQP1_1-10-1 and AQP10_10-1-10. (A) Si influx. Oocytes incubated in medium B2 for 90 min at room temperature were assayed for Si content. They were from 10 oocytes among three to four experiments. According to this presentation, a mean of 1 indicates no n-fold differences (Δ) in Si influx. (B) Water permeability. Oocytes incubated in plain water were assayed for volume measurements as described in Materials and methods. They were from three to five oocytes among three experiments. (A and B) Data are expressed as n-fold differences ± SE between AQP1_1-10-1 and AQP1 (left bar) or between AQP10_10-1-10 and AQP10 (right bar) after background subtraction and normalization to channel expression levels. (C) AQP expression at the cell surface (EXPcs). Oocytes incubated in medium B2 for 90 min were lysed for Western blot analyses using specific anti-AQP Abs, and the signals obtained were quantified through densitometry. Data are expressed as n-fold differences ± SE between AQP1_1-10-1 and AQP1 (left bar) or between AQP10_10-1-10 and AQP10 (right bar) after background subtraction. They were from three experiments. (A–C) The asterisk is used to indicate that the mean is significantly different statistically (*, P < 0.05) compared with the wild-type channel.