Figure 3.
Figure 3. Cross-plots of fluorescence intensity versus current amplitude for mHCN1-EGFP and spHCN-EGFP channels. (A, left) Macroscopic current of the mHCN1-EGFP channel recorded with 3 µM cAMP in the bath. A voltage step from 0 to −120 mV was used for channel activation. Tail current was recorded at −40 mV. (B, left) Macroscopic current of spHCN-EGFP channel recorded with 30 µM cAMP in the bath. A voltage step from 0 to −120 mV was used for channel activation. Tail current was recorded at 40 mV. (A and B, right) The corresponding brightfield and fluorescence images. (C) Cross-plots of fluorescence intensity versus current amplitude for the mHCN1-EGFP channel. Linear fit statistics: Pearson correlation coefficient, 0.973. (D) Cross-plots of fluorescence intensity versus current amplitude for the spHCN-EGFP channel. Linear fit statistics: Pearson correlation coefficient, 0.859. Green curves show the confidence bands (95%).

Cross-plots of fluorescence intensity versus current amplitude for mHCN1-EGFP and spHCN-EGFP channels. (A, left) Macroscopic current of the mHCN1-EGFP channel recorded with 3 µM cAMP in the bath. A voltage step from 0 to −120 mV was used for channel activation. Tail current was recorded at −40 mV. (B, left) Macroscopic current of spHCN-EGFP channel recorded with 30 µM cAMP in the bath. A voltage step from 0 to −120 mV was used for channel activation. Tail current was recorded at 40 mV. (A and B, right) The corresponding brightfield and fluorescence images. (C) Cross-plots of fluorescence intensity versus current amplitude for the mHCN1-EGFP channel. Linear fit statistics: Pearson correlation coefficient, 0.973. (D) Cross-plots of fluorescence intensity versus current amplitude for the spHCN-EGFP channel. Linear fit statistics: Pearson correlation coefficient, 0.859. Green curves show the confidence bands (95%).

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