Figure 2.
Figure 2. PCF recordings of the mHCN2-EGFP channel and cross-plots of fluorescence intensity versus current amplitude. (A, left) Macroscopic current recorded with 3 µM cAMP in the bath (red trace) and a voltage step from −40 to −140 mV. (right) The corresponding brightfield and fluorescence images. (B, left) Histogram of the pixel intensities of the whole image collected with four different exposure durations. Notice longer exposure durations, 100 and 200 ms, lead to saturated pixels. (right) Total fluorescence intensity from the membrane patch (green) and the background (blue). (C) Cross-plots of fluorescence intensity versus current amplitude. Linear fit statistics: Pearson correlation coefficient, 0.907. Green curves show the confidence bands (95%).

PCF recordings of the mHCN2-EGFP channel and cross-plots of fluorescence intensity versus current amplitude. (A, left) Macroscopic current recorded with 3 µM cAMP in the bath (red trace) and a voltage step from −40 to −140 mV. (right) The corresponding brightfield and fluorescence images. (B, left) Histogram of the pixel intensities of the whole image collected with four different exposure durations. Notice longer exposure durations, 100 and 200 ms, lead to saturated pixels. (right) Total fluorescence intensity from the membrane patch (green) and the background (blue). (C) Cross-plots of fluorescence intensity versus current amplitude. Linear fit statistics: Pearson correlation coefficient, 0.907. Green curves show the confidence bands (95%).

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