Figure 1.

Spectral absorbance of rhodopsin and its photoproducts Meta II and Meta III. (A) Transverse polarization absorbance spectra of rhodopsin, Meta II, and Meta III from a typical MSP recording. Raw spectra were recorded from dark-adapted WT mouse retina (black), immediately after near-complete bleaching of the visual pigment with 500-nm light (purple), and 6 min after bleaching (blue). Dashed line represents spectrum after 60 min when all photoproducts have decayed. (B) Mean ± SEM normalized spectrum of Meta II (n = 10; λmax = 384 nm). (C) Mean ± SEM normalized spectrum of Meta III (n = 13; λmax = 472 nm). All recordings were made at 37°C.

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