Net charge versus pH plot and hydrophobicity plot of calcins. (A) Net charge versus pH plot of calcins. The theoretical isoelectric points of calcins are highly basic with values ranging from 9.3 (vejocalcin) to 10.1 (urocalcin), whereas the net charges at pH 7.0 are positive for all calcins and vary from 5.8 (vejocalcin) to 8.7 (urocalcin). (B) Hydrophobicity plot of calcins. The ratio of hydrophilic residues (green) of calcins is between 52% (imperacalcin and hemicalcin) and 66% (hadrucalcin), indicating high solubility in water for all calcins.