Figure 1. Purification and characterization of vejocalcin. (A) 1.5 mg of soluble venom from V. mexicanus was separated by HPLC using a semi-preparative C18 reverse-phase column, eluted with a linear gradient from solution A to 60% solution B, run for 60 min. The fraction labeled with the asterisk was rechromatographed in an analytical C18 reverse-phase column and run from solution A to 40% solution B in 60 min. (B) The component with retention time of 28.09 min (marked with an asterisk) is vejocalcin. (C) The complete amino acid sequence of vejocalcin was obtained by a combination of direct Edman degradation and mass spectrometry, as described in Materials and methods.
Figure 1.

Purification and characterization of vejocalcin. (A) 1.5 mg of soluble venom from V. mexicanus was separated by HPLC using a semi-preparative C18 reverse-phase column, eluted with a linear gradient from solution A to 60% solution B, run for 60 min. The fraction labeled with the asterisk was rechromatographed in an analytical C18 reverse-phase column and run from solution A to 40% solution B in 60 min. (B) The component with retention time of 28.09 min (marked with an asterisk) is vejocalcin. (C) The complete amino acid sequence of vejocalcin was obtained by a combination of direct Edman degradation and mass spectrometry, as described in Materials and methods.

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