Expression of mRNA for atrophy and protein degradation–associated factors. Larvae (4 dpf) injected with control morpholinos (open bars) or with morpholinos for mybpc-1 (hatched bars) and mybpc-2b (black bars). The qPCR data for muscle-specific RING finger proteins (MuRF1–3), microtubule-associated protein 1 light chain 3B (LCB3), sequestosome 1 (SQSTM1), and myogenin are expressed relative to β-actin levels; n = 3–4 in each group. Statistical analyses (ANOVA) are indicated: *, P < 0.05; **, P < 0.01; ***, P < 0.001. (inset) Acridine orange staining of a control (left)- and mybpc-2b (right)-injected larvae at 1 dpf. Arrows indicate muscle regions with increased apoptosis. Bar, 50 µm.