Figure 4.
Figure 4. Cellular distribution of RyR1 and Cav1.1 in differentiated orbicularis oculi–derived myotubes. Human myotubes were visualized with an A1R confocal microscope equipped with a CFI Apo TIRF 100× objective (1.49 NA) and stained as described in Materials and methods. Top panels show orbicularis oculi, and bottom panels show EOMs. (A and E) Anti-Cav1.1 (green). (B and F) Anti-RyR1 (red). (C and G) Merged image of anti-RyR1, anti-Cav1.1, and DAPI (blue); orange pixels show codistribution of RyR1 and Cav1.1. (D and H) Anti-Cav1.2 (green). Bars, 20 µm.

Cellular distribution of RyR1 and Cav1.1 in differentiated orbicularis oculi–derived myotubes. Human myotubes were visualized with an A1R confocal microscope equipped with a CFI Apo TIRF 100× objective (1.49 NA) and stained as described in Materials and methods. Top panels show orbicularis oculi, and bottom panels show EOMs. (A and E) Anti-Cav1.1 (green). (B and F) Anti-RyR1 (red). (C and G) Merged image of anti-RyR1, anti-Cav1.1, and DAPI (blue); orange pixels show codistribution of RyR1 and Cav1.1. (D and H) Anti-Cav1.2 (green). Bars, 20 µm.

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