Figure 2.
Figure 2. Modified pHo sensitivity of W207A mutant hHV1. Families of proton currents at several pHo in a cell with pHi 7.0 are illustrated (A–D). Pulses were applied in 10-mV increments up to the voltage indicated, from a holding potential of −60 (A) or −40 mV (B–D). (E) Proton current amplitudes (IH) from the cell in A–D were obtained by fitting the rising current with a single exponential and extrapolating to infinite time. (F) Proton conductance (gH) was calculated from the currents in E using Vrev measured in each solution.

Modified pHo sensitivity of W207A mutant hHV1. Families of proton currents at several pHo in a cell with pHi 7.0 are illustrated (A–D). Pulses were applied in 10-mV increments up to the voltage indicated, from a holding potential of −60 (A) or −40 mV (B–D). (E) Proton current amplitudes (IH) from the cell in A–D were obtained by fitting the rising current with a single exponential and extrapolating to infinite time. (F) Proton conductance (gH) was calculated from the currents in E using Vrev measured in each solution.

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