Figure 2.
Figure 2. Effects of a low-Na diet on processed ENaC subunits at the cell surface. (A) For αENaC, the fully processed form was identified as the 30-kD cleaved species, which increased with Na depletion. Two gels from different experiments are shown. Noncontiguous portions of the same gel are compared, with intermediate portions excised for clarity. No further image processing was performed. (B) For βENaC, the EndoH (EH)-resistant form was strongly increased, whereas the EndoH-sensitive form was not significantly affected by Na depletion. (C) For γENaC, the EndoH-resistant form was strongly increased, whereas the EndoH-sensitive form was reduced by Na depletion. (D) Quantitation of the results in A–C. The cleaved form of αENaC and EndoH-resistant forms of βENaC and γENaC increased to similar extents with Na depletion (∼3.5-fold over control levels). Results represent mean ± SEM of four independent experiments. *, P < 0.05 by t test.

Effects of a low-Na diet on processed ENaC subunits at the cell surface. (A) For αENaC, the fully processed form was identified as the 30-kD cleaved species, which increased with Na depletion. Two gels from different experiments are shown. Noncontiguous portions of the same gel are compared, with intermediate portions excised for clarity. No further image processing was performed. (B) For βENaC, the EndoH (EH)-resistant form was strongly increased, whereas the EndoH-sensitive form was not significantly affected by Na depletion. (C) For γENaC, the EndoH-resistant form was strongly increased, whereas the EndoH-sensitive form was reduced by Na depletion. (D) Quantitation of the results in A–C. The cleaved form of αENaC and EndoH-resistant forms of βENaC and γENaC increased to similar extents with Na depletion (∼3.5-fold over control levels). Results represent mean ± SEM of four independent experiments. *, P < 0.05 by t test.

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