CRAC channel CDI phenotypes for mutations of the triple basic ring. All currents were recorded as described in Fig. 3. Representative currents are shown for WT Orai1 (A, same traces shown in Fig. 3 A), and Orai1 point mutants R83E (B), R83N (C), K87E (D), and R91E (E), with the extent of CDI summarized in F. Each point represents the mean ± SEM for n = 6–10 cells.