Sequence alignments of the S4–S5 linker (A) and post-S6 (B) for all human EAG family channels, Drosophila and Nematostella Elk, and human Kv1.2 and KCNQ1. Basic residues Lys and Arg are shaded blue, and His is shaded green. Acidic residues are shaded red, and other positions with general conservation are shaded gray. Elk1 R347, R475, and R479 are marked with red stars; Kv1.2 and KCNQ1 residues required for PIP₂ modulation are outlined in red. Domain boundaries are indicated below the alignments, and sequence positions are indicated at the right margin. GenBank sequence accession numbers for EAG family channels are given in the legend to Fig. 6, and are NM_004974 and NM_000218 for Kv1.2 and KCNQ1, respectively.