Permeability properties of mutant connexins. (A) Summary of single-channel permeability (Pγ) for mutants and WT Cx40 gap junction channels to LY and EtBr: A96S (pink bar), M163V (yellow bar), G38D (green bar), hCx40WT (gray bar), and rCx40WT (black bar). Refer to Results and Table 1 for further information. (B) Permeability of fluorescent probes relative to K⁺ ions for mutants and WT Cx40: A96S (pink bar), M163V (yellow bar), G38D (green bar), hCx40WT (gray bar), and rCx40WT (black bar). The bars represent probe/K⁺ ratios plotted on a log scale. See Table 1 and Results for details. Error bars represent means ± SEM.