CRAC channel CDI phenotypes for mutations at Orai1 position W76. All currents were recorded and analyzed as described for Fig. 3. Representative currents are shown for WT Orai1 (A, same traces shown in Fig. 3 A) and Orai1 point mutants. W76A (B), W76I (C), W76F (D), W76E (E), and W76K (F), with the extent of CDI for this series summarized in G. Fast (H) and slow (I) time constants from biexponential fits are plotted against test potential; amplitudes of fast and slow components are shown in Fig. S3 (C and D). Each point on the summary graphs represents the mean ± SEM for n = 4–6 cells. Representative currents for additional mutations on the summary graphs are shown in Fig. S6.