Figure 2.
Figure 2. Examples of motion of single QD-MLCK and QD-N75 molecules on actin. (A) Montage of QD-MLCK (green) moving on an MLCK-induced actin bundle (red) showing the position of QD at 0.4-s intervals up to 7.4 s. Each image is 53 × 29 pixels or 5.6 × 3.1 µm. Below in greyscale is a kymograph showing position (vertical axis) with time (horizontal axis) generated by selecting a line following the actin filament, starting at the right, to match the X–Y trajectory shown below of the same QD. Blurry periods may be caused by the QD approaching the edge of the TIRF field. (B) Montage of QD-N75 (green) moving on a HASMC stress fiber (red) showing the position of QD at 0.2-s intervals up to 9.8 s. Each image is 20 × 26 pixels or 2.1 × 2.8 µm. Montages were prepared with ImageJ, and X–Y trajectories were prepared with the SpotTracker 2-D plugin for ImageJ.

Examples of motion of single QD-MLCK and QD-N75 molecules on actin. (A) Montage of QD-MLCK (green) moving on an MLCK-induced actin bundle (red) showing the position of QD at 0.4-s intervals up to 7.4 s. Each image is 53 × 29 pixels or 5.6 × 3.1 µm. Below in greyscale is a kymograph showing position (vertical axis) with time (horizontal axis) generated by selecting a line following the actin filament, starting at the right, to match the X–Y trajectory shown below of the same QD. Blurry periods may be caused by the QD approaching the edge of the TIRF field. (B) Montage of QD-N75 (green) moving on a HASMC stress fiber (red) showing the position of QD at 0.2-s intervals up to 9.8 s. Each image is 20 × 26 pixels or 2.1 × 2.8 µm. Montages were prepared with ImageJ, and X–Y trajectories were prepared with the SpotTracker 2-D plugin for ImageJ.

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