Role of integrins in the cyclic transmural pressure–induced vasodilation. (A) RGD peptide (GRGDSP) incubated with the vessel segments for 60 min prevented the vasodilation induced by the cyclic transmural pressure. (B) Control RGE peptide (GRGESP) did not affect the cyclic transmural pressure–induced vasodilation. (C) Function-blocking integrin α₅β₁ or α_vβ₃ antibodies compromised the cyclic transmural pressure–induced vasodilation. The top two panels represent the coronary arteries, and bottom two panels represent the skeletal muscle arteries. The vessel segments were incubated with function-blocking integrin α₅β₁ and α_vβ₃ antibodies, which were continuously administrated into lumen with a microsyringe pump.