Figure 1. The Kv2.1 V378A mutant produces voltage-activated currents. Whole-cell patch-clamp recordings from transiently transfected CHO-K1 cells. Currents are responses to voltage steps from a holding potential of −100 mV. (A) Kv2.1 wild-type currents were stepped for 100 ms to −80, −40, 0, 40, or 80 mV, and then returned to −100 mV. (B) Kv2.1 V378A currents elicited by the same protocol as A. (C) Conductance–voltage relation of Kv2.1 wild-type (open circles) and V378A (filled circles) measured from tail currents after 100-ms depolarizing steps to the indicated voltage. Lines are fourth-power Boltzmann fits. Wild-type Vmid = 8 mV and z = 0.9 e+; V378A Vmid = −21 mV and z = 1.1 e+. (D) Current from wild-type Kv2.1. 100-ms step to 40 mV. Thin trace, control; thick trace, 100 nM GxTX. (E) Kv2.1 V378A currents, with same protocols as D. (F) Summary of inhibition by 100 nM GxTX at 40 mV. Wild-type, n = 4; V378A, n = 3. Error bars represent means ± SEM.
Figure 1.

The Kv2.1 V378A mutant produces voltage-activated currents. Whole-cell patch-clamp recordings from transiently transfected CHO-K1 cells. Currents are responses to voltage steps from a holding potential of −100 mV. (A) Kv2.1 wild-type currents were stepped for 100 ms to −80, −40, 0, 40, or 80 mV, and then returned to −100 mV. (B) Kv2.1 V378A currents elicited by the same protocol as A. (C) Conductance–voltage relation of Kv2.1 wild-type (open circles) and V378A (filled circles) measured from tail currents after 100-ms depolarizing steps to the indicated voltage. Lines are fourth-power Boltzmann fits. Wild-type Vmid = 8 mV and z = 0.9 e+; V378A Vmid = −21 mV and z = 1.1 e+. (D) Current from wild-type Kv2.1. 100-ms step to 40 mV. Thin trace, control; thick trace, 100 nM GxTX. (E) Kv2.1 V378A currents, with same protocols as D. (F) Summary of inhibition by 100 nM GxTX at 40 mV. Wild-type, n = 4; V378A, n = 3. Error bars represent means ± SEM.

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