Figure 5.
Figure 5. Introduction of alanines at Rem positions R200, L227, and H229 ablates the ability of V-Rem to inhibit L-type Ca2+ current conducted by CaV1.3/β1a/α2δ-1 channels expressed in tsA201 cells. Representative L-type currents are shown for tsA201 cells coexpressing CaV1.3/β1a/α2δ-1 and V-Rem (A), V-Rem AAA (B), or YFP (C). Current families shown were evoked by 50-ms steps from −80 to −40 through 60 mV in 10-mV increments. Current amplitudes were normalized by linear cell capacitance (pA/pF). (D) I-V relationships are shown. (E) Confocal images confirming successful heterologous expression of V-Rem and V-Rem AAA in tsA201 cells are shown. Bars, 10 µm. Error bars represent ±SEM.

Introduction of alanines at Rem positions R200, L227, and H229 ablates the ability of V-Rem to inhibit L-type Ca2+ current conducted by CaV1.3/β1a/α2δ-1 channels expressed in tsA201 cells. Representative L-type currents are shown for tsA201 cells coexpressing CaV1.3/β1a/α2δ-1 and V-Rem (A), V-Rem AAA (B), or YFP (C). Current families shown were evoked by 50-ms steps from −80 to −40 through 60 mV in 10-mV increments. Current amplitudes were normalized by linear cell capacitance (pA/pF). (D) I-V relationships are shown. (E) Confocal images confirming successful heterologous expression of V-Rem and V-Rem AAA in tsA201 cells are shown. Bars, 10 µm. Error bars represent ±SEM.

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