Ca2+ spark frequency in intact versus permeabilized myocytes after myocardial infarction. (A; top) Line-scan images obtained in intact myocytes from sham (left) or from PMI (right) mice. (Bottom) Bar graph showing the Ca2+ spark frequency in intact cells from sham (n = 59 from five animals) and PMI mice (n = 41 from seven animals). (B; top) Line-scan images obtained in permeabilized at 50 nmol/liter [Ca2+]i myocytes from sham (left) or from PMI (right) mice. (Bottom) Bar graph showing the increased Ca2+ spark frequency in permeabilized cells from PMI mice (n = 27 cells from three animals) compared with myocytes from sham mice (n = 26 cells from three animals). ***, P < 0.001 versus myocytes from sham mice. Error bars represent the SEM.