Sequence diversity in the TRP channel superfamily. (A) The sequence analysis workflow followed in this study. The top row shows the protocol for sequence collection and curation (script in red), whereas the bottom row shows the analyses performed on the final full MSA (script in purple). This workflow was applied both to the TRP and Kv channel superfamily. Details of procedures are available in Materials and methods. (B) Histograms of pairwise sequence identity for the TRP channel superfamily. Only the aligned TM region was used and the identity was calculated for all pairs of sequences in each subfamily. (C) Phylogenetic tree of the full TRP channel MSA inferred with FastTree2. Annotated sequences were used to identify branches corresponding to known TRP subfamilies.