Figure 7.
![Figure 7. Apparent K+ affinity of the Na,K-ATPase α2 isoform measured in membranes prepared from wild-type and skα2−/− mice. (A) Mean ATPase activity versus [K+] for skeletal muscle membranes prepared from five wild-type (□) and four skα2−/− (○) mice. Solid lines are fits of the data to the Hill equation, with the Hill coefficient constrained to 1.68. (B) Mean fitted K1/2,K values for wild-type and skα2−/− muscle preparations. *, significant difference at P < 0.04. Na+ concentration was fixed at 80 mM, and K+ concentration was varied from 0 to 20 mM by equimolar replacement with choline chloride. Temperature, 22°C. Error bars represent mean ± SEM.](https://cdn.rupress.org/rup/content_public/journal/jgp/146/4/10.1085_jgp.201511407/5/jgp_201511407_fig7.jpeg?Expires=1767767571&Signature=DtcB0kVsgN7vhlhqtzpVNgjW3o9xEmBW1crtV5jFpDhZgakj3EgmPsHAWY1nVA7jyeRbHsT2x2pRwmzApl6SxjPoaOLqGj5v22KbcHxlhFvU9MXykVfviEDym0BVcX7z3UDSdF9EVuYYAFrHZA3BrBvqjrLpSsJ7DT8NifwMmWOJeWyueGxdJxIuUj1Njd8h~SpSMKh-m9Yo2AZ7Ro2hGilsRUd8rUWIWCg69Wvt9lfZoSIvp9yaOyWvX98pcAAYZIqZ1s0MTl4nHQK9yIPBLgSscft0fsumBojZLRaYTaOxoufIVNneJj0NVkT0xSjDdhgM1VskxUcu6dmveZ-9Jg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Apparent K+ affinity of the Na,K-ATPase α2 isoform measured in membranes prepared from wild-type and skα2−/− mice. (A) Mean ATPase activity versus [K+] for skeletal muscle membranes prepared from five wild-type (□) and four skα2−/− (○) mice. Solid lines are fits of the data to the Hill equation, with the Hill coefficient constrained to 1.68. (B) Mean fitted K1/2,K values for wild-type and skα2−/− muscle preparations. *, significant difference at P < 0.04. Na+ concentration was fixed at 80 mM, and K+ concentration was varied from 0 to 20 mM by equimolar replacement with choline chloride. Temperature, 22°C. Error bars represent mean ± SEM.
