Comparison of TRPM3 recovery by different PIPs. (A) Representative time course showing variable recovery of TRPM3 activity in an inside-out patch upon application of the indicated diC8 PIPs at a concentration of 50 µM. (B) Statistical analysis of the current recovery at +120 mV by 50 µM of the indicated diC8 PIPs. The dotted line indicates the mean recovery obtained with 2 mM ATP. Numbers of individual membrane patches are in parentheses. (C) Representative I-V curves showing the current response to 25 µM diC16 PI(4,5)P₂. (D) Dose dependence of the effect of diC8 PI(4,5)P₂, diC8 PI(3,4,5)P₃ and diC16 PI(4,5)P₂. Currents were normalized to the peak current upon excision (indicated by the gray line). n = 2–21/data point. Solid lines represent a logistic function fitted to the data. Since saturation could not be obtained at the highest testable concentrations, reliable EC₅₀ values could not be obtained. The inset shows a magnification of the lower part of the dose–response graph, allowing estimation of the concentrations required to obtain 100% current recovery.