Effect of voltage-sensitive 5-phosphatases on TRPM3 whole-cell currents. (A) Representative time course of whole-cell currents measured at +120 mV in a cell coexpressing TRPM3 and Ci-VSP, illustrating the effect of changing holding potential from −70 to +90 mV (white and gray background, respectively). (B) I-V traces at different time points indicated in A. (C and D) Same as in A and B, except for a cell transfected with inactive mutant Ci-VSP-C363S. (E) Representative time course of whole-cell currents measured at +40 mV in a cell coexpressing TRPM3 and Dr-VSP, illustrating the effect of changing holding potential from −60 to +100 mV (white and gray background, respectively) using an intracellular solution containing 4 mM ATP. (F) Same as E, but using an intracellular solution containing 4 mM AMPPNP. (G) Comparison of relative currents measured at +120 mV and with a depolarizing holding potential (+90 mV) in cells expressing Ci-VSP and Ci-VSP-C363S. Currents were normalized to the currents measured at a −70-mV holding potential. P-values were obtained by unpaired Student’s t test. (H) Comparison of relative currents measured at +40 mV and with a depolarizing holding potential (+100 mV) in cells expressing TRPM3 without (control) or with Dr-VSP, for the conditions described in E and F. Current were normalized to the currents measured at a −60-mV holding potential. P-values were obtained by one-way ANOVA, with Bonferroni post-hoc.