ATP-induced recovery of TRPM3 requires PI-kinase activity. (A) Representative time course showing the lack of current recovery (<5%) upon ATP application in Mg²⁺-free solution. (B) Representative time course showing the lack of current recovery (<5%) upon application of AMPPCP (2 mM). (C and D) Representative time courses showing the rapid inhibition of TRPM3 activity by the PIP scavengers PLL (50 µg/ml) and Neomycin (5 mM) applied to the cytoplasmic side of inside-out membrane patches. (E) Statistical analysis of current inhibition at +120 mV by the various PIP scavenging agents. Values are given as percentage of the ATP-induced current recovery just before the application of the compound. P-values were determined by using one sample Student’s t test. (F and G) Representative time courses showing the impaired ATP-induced recovery of TRPM3 activity in the presence of the PI-kinase inhibitors wortmannin and LY294,002. (H) Comparison of the ATP-induced current recovery in the absence and presence of PI-kinase inhibitors. P-values were obtained by one-way ANOVA, with Bonferroni post-hoc test to compare with the inhibitor-free condition.