Figure 4.
PI-PLC eliminates the effect of MgATP. Excised inside-out patch measurements have been performed on hTRPM3-expressing Xenopus oocytes with 100 µM PregS in the patch pipette as described in Materials and methods; data are plotted at 100 and −100 mV. (A and B) Representative traces for the effects of MgATP in the absence and presence of PI-PLC; the applications of 2 mM MgATP, 1 U/ml PI-PLC, and 25 µM diC8 PI(4,5)P2 are indicated by the horizontal lines. Vehicle denotes standard bath solution with 0.5% glycerol. (C) Summary of the data for control and PI-PLC–treated patches (n = 6). Error bars represent SEM. **, P < 0.01.
