The effects of 3-phosphorylated phosphoinositides on hTRPM3. (A–C) Representative traces at 100 and −100 mV; experiments were performed on hTRPM3-expressing Xenopus oocytes with 100 µM PregS in the patch pipette as described in Materials and methods. The establishment of the inside-out (i/o) configuration is indicated with an arrow. The applications of 25 µM diC₈ PI(4,5)P₂, 25 µM diC₈ PI(3,4)P₂, 25 µM diC₈ PI(3,5)P₂, and 25 µM diC₈ PI(3,4,5)P₃ are indicated with the horizontal lines. (D) Statistical summary of current amplitudes at 100 mV; the data are normalized to the TRPM3 current evoked by diC₈ PI(4,5)P₂ (n = 6–7). (E) Representative measurement for a concentration–response relationship for PI(3,4,5)P₃. (F) Statistical summary compared with PI(4,5)P₂, which is replotted from Fig. 1 F (dashed line). Error bars represent SEM.