Suppression of calcium waves by modulation of the spontaneous opening transition of the RyR. (A–C) Typical simulations of calcium release events at cytosolic [Ca²⁺] of 0.05 µM in control (A), after a threefold decrease of f_Ca (B), and when a threefold decrease of f_Ca was combined with a threefold increase of K_O00 (C). (D) Frequency of triggered sparks at [Ca²⁺] of 0.05 µM under control conditions and when the indicated RyR gating parameters were changed in the direction supporting wave formation (33% f_Ca, 33% K_Ca, 300% K_Mg, and 200% p_dis). Gray columns indicate results of simulation with a control value of K_O00, whereas the results for K_O00 increased to 300% of control are shown as black columns. Significant differences between groups are shown by horizontal black lines (one-way ANOVA with Bonferroni correction; P < 0.05). Error bars represent mean ± SEM.