Functional expression and K⁺_e inhibition of E284C, D286C, and E295C currents. (A) Representative current traces of E284C, D286C, and E295C with different K⁺_e. The currents were recorded after 48–96 h of transfection using the pulse protocol described in Fig. 2 C. (B) The relationships between the relative current magnitude and the K⁺_e of E284C (n = 8), D286C (n = 6), and E295C (n = 6). The experimental data of the three mutant channels were nearly superimposable on the WT hK_v7.1 data. They were all at odds with predicted values by the GHK equation.