Figure 5.
Figure 5. Effects of NO3− on single-channel kinetics of ΔR-CFTR and D1370N/ΔR-CFTR. (A) Modulation of ΔR-CFTR gating by NO3−. Because the activity of ΔR-CFTR is independent of phosphorylation, only ATP is included in the bath. (B) Summary of the effect of NO3− on the single-channel kinetics of ΔR-CFTR. Po, τo, and τc: 0.44 ± 0.02, 342 ± 28 ms, and 432 ± 39 ms for Cl−; and 0.68 ± 0.02, 751 ± 138 ms, and 336 ± 55 ms for NO3− (n = 15). (C) NO3− increases the Po of a hydrolysis-deficient mutant CFTR (D1370N). (D) Microscopic kinetic parameters for D1370N/ΔR-CFTR in the presence of bath Cl− or NO3−. Po, τo, and τc: 0.41 ± 0.02, 544 ± 45 ms, and 759 ± 48 ms for Cl−; and 0.77 ± 0.02, 1,889 ± 278 ms, and 508 ± 71 ms for NO3− (n = 10). The error bars represent the SEM of the mean.

Effects of NO3− on single-channel kinetics of ΔR-CFTR and D1370N/ΔR-CFTR. (A) Modulation of ΔR-CFTR gating by NO3−. Because the activity of ΔR-CFTR is independent of phosphorylation, only ATP is included in the bath. (B) Summary of the effect of NO3− on the single-channel kinetics of ΔR-CFTR. Po, τo, and τc: 0.44 ± 0.02, 342 ± 28 ms, and 432 ± 39 ms for Cl−; and 0.68 ± 0.02, 751 ± 138 ms, and 336 ± 55 ms for NO3− (n = 15). (C) NO3− increases the Po of a hydrolysis-deficient mutant CFTR (D1370N). (D) Microscopic kinetic parameters for D1370N/ΔR-CFTR in the presence of bath Cl− or NO3−. Po, τo, and τc: 0.41 ± 0.02, 544 ± 45 ms, and 759 ± 48 ms for Cl−; and 0.77 ± 0.02, 1,889 ± 278 ms, and 508 ± 71 ms for NO3− (n = 10). The error bars represent the SEM of the mean.

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