Figure 3.
Figure 3. Effect of cytoplasmic replacement of Cl− with NO3− on WT-CFTR gating. (A) Single-channel current traces of WT-CFTR in the presence of bath Cl− or NO3−. CFTR in an inside-out patch was activated by PKA and ATP before the bath solution was switched to one with 2 mM ATP in Cl− or NO3−. Holding potential, −50 mV. (B) Single-channel kinetic parameters of WT-CFTR in the presence of bath Cl− or NO3−. Po, open time constant (τo), and closed time constant (τc): 0.50 ± 0.01, 311 ± 27 ms, and 315 ± 7 ms for Cl−; and 0.74 ± 0.02, 709 ± 132 ms, and 240 ± 19 ms for NO3− (n = 4). The error bars represent the SEM of the mean.

Effect of cytoplasmic replacement of Cl− with NO3− on WT-CFTR gating. (A) Single-channel current traces of WT-CFTR in the presence of bath Cl− or NO3−. CFTR in an inside-out patch was activated by PKA and ATP before the bath solution was switched to one with 2 mM ATP in Cl− or NO3−. Holding potential, −50 mV. (B) Single-channel kinetic parameters of WT-CFTR in the presence of bath Cl− or NO3−. Po, open time constant (τo), and closed time constant (τc): 0.50 ± 0.01, 311 ± 27 ms, and 315 ± 7 ms for Cl−; and 0.74 ± 0.02, 709 ± 132 ms, and 240 ± 19 ms for NO3− (n = 4). The error bars represent the SEM of the mean.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal