Molecular BK model and MD analysis. (A) Model built for open BK WT channel pore region (green balls are K⁺ and blue balls are Cl⁻). (B) Side view of the hydrophobic ring formed by the F380 and L377 residues in BK WT (one subunit is transparent to better view). (C–E) Top view of the hydrophobic ring formed by the 380 and 377 residues in BK WT, F380A, and F380W, respectively. (F) Interaction energy between L377 and 380 residues (WT is considered a reference).