Single evoked APs in CCs without β2 subunits exhibit slowed repolarization and an absence of AHP. (A) The indicated injected current pulse (5 ms for 150 [or 250] pA) was used to elicit single APs in CCs in slices. For each cell, an averaged AP was generated from 3–10 individual APs. The averaged AP from each cell was then averaged with those from other cells, with n as indicated. APs are from sets of BK_i cells stimulated either with a 150-pA pulse (blue trace) or 250 pA pulse (black). The voltage reached during the AHP was significantly more different with the 250-pA pulse. (B) APs are compared for sets of β2 KO cells stimulated either with a 150 (black)- or 250-pA (red) pulse. The 250-pA AP exhibits a larger peak amplitude. (C) APs are compared for BK_i and β2 KO cells stimulated with a 150-pA pulse. β2 KO cells exhibit a smaller peak amplitude and pronounced depolarizing afterpotential. (D) APs are compared for BK_i and β2 KO cells stimulated with a 250-pA pulse. The depolarizing afterpotential is even more pronounced. (E) APs are compared for BK_i and BK_s cells, showing a smaller peak AP amplitude and reduced AHP in the BK_s cells. (F) APs are compared for BK_i and Slo1 KO CCs, showing a larger peak amplitude and slower repolarization in the Slo1 KO cells.