Figure 7. Effects of NPPB, 3NB, and 3PP on WT CFTR microscopic steady-state gating parameters. (A) Currents from single WT CFTR channels at −80 mV in 2 mM ATP (top), 2 mM ATP + 32 mM 3NB (middle), and 2 mM ATP + 20 mM 3PP (bottom); bandwidth, 50 Hz. (B) Cartoon gating model; opening rate (top purple arrow) and closing rate (bottom purple arrow) are given by 1/τib and 1/τb, respectively. (C–E) Open probabilities (C) and mean burst (τb; D) and mean interburst (τib; E) durations in 2 mM ATP (gray), 2 mM ATP + 210 µM NPPB (brown), 2 mM ATP + 32 mM 3NB (blue), and 2 mM ATP + 20 mM 3PP (red). The data for NPPB (brown bars) are replotted from Csanády and Töröcsik (2014) and reflect values measured at 60 mV. Mean ± SEM is shown.
Figure 7.

Effects of NPPB, 3NB, and 3PP on WT CFTR microscopic steady-state gating parameters. (A) Currents from single WT CFTR channels at −80 mV in 2 mM ATP (top), 2 mM ATP + 32 mM 3NB (middle), and 2 mM ATP + 20 mM 3PP (bottom); bandwidth, 50 Hz. (B) Cartoon gating model; opening rate (top purple arrow) and closing rate (bottom purple arrow) are given by 1/τib and 1/τb, respectively. (C–E) Open probabilities (C) and mean burst (τb; D) and mean interburst (τib; E) durations in 2 mM ATP (gray), 2 mM ATP + 210 µM NPPB (brown), 2 mM ATP + 32 mM 3NB (blue), and 2 mM ATP + 20 mM 3PP (red). The data for NPPB (brown bars) are replotted from Csanády and Töröcsik (2014) and reflect values measured at 60 mV. Mean ± SEM is shown.

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