Figure 6. Effects of 3NB and 3PP on macroscopic closing rate of WT CFTR. (A and B) Macroscopic WT CFTR currents at −80 mV, elicited by brief applications of 2 mM ATP (gray bars) in the absence of drug or in the presence of either 32 mM 3NB (A, blue bar) or 20 mM 3PP (B, red bar). All four current decay time courses after ATP removal were fitted by single exponentials (colored lines), and time constants are indicated. (C) Macroscopic closing rates, obtained as the inverses of fitted time constants (see Materials and methods), under control conditions (gray bar) or in the presence of either 32 mM 3NB (blue bar) or 20 mM 3PP (red bar). Mean ± SEM is shown. Cartoon in C (also in Fig. 7 B; Fig. 8, C and G; and Fig. 10) depicts simplified cyclic CFTR gating model: cyan, TMDs; green, NBD1; blue, NBD2; yellow, ATP; red, ADP. Purple arrow highlights the pathway under study.
Figure 6.

Effects of 3NB and 3PP on macroscopic closing rate of WT CFTR. (A and B) Macroscopic WT CFTR currents at −80 mV, elicited by brief applications of 2 mM ATP (gray bars) in the absence of drug or in the presence of either 32 mM 3NB (A, blue bar) or 20 mM 3PP (B, red bar). All four current decay time courses after ATP removal were fitted by single exponentials (colored lines), and time constants are indicated. (C) Macroscopic closing rates, obtained as the inverses of fitted time constants (see Materials and methods), under control conditions (gray bar) or in the presence of either 32 mM 3NB (blue bar) or 20 mM 3PP (red bar). Mean ± SEM is shown. Cartoon in C (also in Fig. 7 B; Fig. 8, C and G; and Fig. 10) depicts simplified cyclic CFTR gating model: cyan, TMDs; green, NBD1; blue, NBD2; yellow, ATP; red, ADP. Purple arrow highlights the pathway under study.

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